Transcriptional Profiling Of Single Skeletal Muscle Mononucleated Cells Coupled With Broadband [...]

Organisation / Company : Università degli Studi di Padova
Research Field : Biological sciences » Biology
Researcher Profile : Recognised Researcher (R2), Leading Researcher (R4), First Stage Researcher (R1), Established Researcher (R3)
Country : Italy
Application Deadline : 17 Jan 2025 - 09 : 00 (UTC)
Type of Contract : To be defined
Job Status : Not Applicable
Funding : Not funded by a EU programme
Research Position : Not related to staff position within a Research Infrastructure
Offer Description : Background : ME / CFS is a chronic disease affecting over 2 million people in Europe and America, characterized by persistent and unexplained post-exertional fatigue.
Its etiology and symptom heterogeneity remain unclear, and no single biomarker or definitive diagnostic sign has been identified.
Aim : The project addresses two key questions: a) What pathways are altered in the diverse cell populations of ME / CFS patient muscles?
b) How do cellular interactions and communication differ between patient and normal muscles?
Methods : The work will integrate impedance sensing, single-cell RNA sequencing, spatial transcriptomics, and pathway analysis to identify differential features, quantitatively explain alterations in ME / CFS patients, and discover new objective markers for diagnosis.
Collaborations : The researcher will work within an international group, collaborating with Lehigh University (USA), and the Universities of Chieti and Padova (Italy).

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