Characterization Of The Phospholipidome Of Biological Samples For The Development And Integrati[]

Organisation/Company: Università degli studi di Bari Research Field: Chemistry Researcher Profile: Recognised Researcher (R2) Leading Researcher (R4) First Stage Researcher (R1) Established Researcher (R3) Country: Italy Application Deadline: 15 Jan 2025 - 23:59 (UTC) Type of Contract: To be defined Job Status: Not Applicable Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No Offer Description The analysis of metabolites, particularly lipids, in physio- and pathological conditions has benefited greatly from the progress in the framework of mass spectrometry techniques. Starting from this context, the phospholipidomic profile will be explored in lipid extracts of cells deriving from different cultures with overexpression of pathological conditions such as cancer by comparing them with control samples. This comparison will allow a preliminary qualitative delineation of aberrant lipids. After preliminary screening by MALDI-MS, the researcher will develop methods based on LC-MS, including reversed-phase and/or hydrophilic-phase liquid chromatography and high-resolution mass spectrometry, to detect lipids and their possible modifications in extracts obtained from cells. The compounds of interest will be recognized by applying a multistage mass spectrometry (MSn) investigation. J-18808-Ljbffr